In 1879 he passed the Entrance Examination and took admission into the Metropolitan Institution (later Vidyasagar College) which was established by Pandit Ishwar Chandra Vidyasagar. At that time the Metropolitan Institution had no science classes or laboratories and Ray attended lectures on physics and chemistry in the Presidency College as an external student. Here he was specially attracted by the chemistry courses of professor Alexander Pedler. While studying for his BA examination, he applied for and was awarded in 1882 one of the two Gilchrist Prize Scholarships after an all-India competitive examination. Without completing the course for his degree, Prafulla Chandra proceeded to Britain and enrolled in the BSc programme of University of Edinburgh where he studied physics, chemistry and biology amongst other subjects. But Ray did not confine his studies to only natural sciences. He also developed a strong interest in history and read books like Rousselet's L'Inde des Rajas, Lanoye's L'Inde contemporaine, Revue dex deux moneds. He also read Fawcett's book on political economy and Essays on Indian Finance. After obtaining his BSc degree from the University of Edinburgh, Ray embarked on his doctoral thesis (DSc) in the same university and completed his doctorate in 1887. He was awarded the Hope Prize which allowed him to work on his research for a further period of one year after completion of his doctorate. His thesis title was "Conjugated Sulphates of the Copper-magnesium Group: A Study of Isomorphous Mixtures and Molecular Combinations". While a student he was elected Vice-President of the University of Edinburgh Chemical Society in 1888.
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